STUDIES ON BLOOD GROUP SUBSTANCES .2. COUPLING OF BLOOD GROUP SUBSTANCE A TO HYDROXYL-CONTAINING MATRICES INCLUDING AMINOETHYL CELLULOSE AND AGAROSE

1. 1. Fully reversible and highly stable immunosorbents have been prepared by coupling of blood group substance A to agarose beads and aminoethyl cellulose using activation of carbohydrate with CNBr. 2. 2. Purification of anti-A phytohemagglutinin from Vicia cracca and human anti-A serum antibody by means of A-substance-agarose is reported. 3. 3. The amino group-carbohydrate linkage formed after activation of carbohydrate with CNBr has been found to be stable between pH 2 and 12. 4. 4. Due to their ease of preparation and the mild conditions required for their preparation, similar adsorbents may be of general value in immunochemistry. © 1969.