INTERFERENCE OF INCOMBUSTIBLE PHYTATES IN ANALYSIS OF PLANT PHOSPHOLIPIDS ON LATROSCAN CHROMARODS

被引:14
作者
ACKMAN, RG
WOYEWODA, AD
机构
[1] Department of Fisheries and Oceans, Halifax, NS, B3J 2S7, P.O. Box 550
关键词
D O I
10.1093/chromsci/17.9.514
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The thin layer chromatographic separation of lipids on Chromarods, followed by measurement of organic carbon in the latroscan TH-10 Mark II Analyser, normally leaves the Chromarods ready for immediate reuse. However, a polar lipid fraction derived from rapeseeds included a component that resisted complete combustion on the Chromarod-S (silicic acid), although sterols, triglycerides, and phospholipids left no detectable residue. The component was mobile in organic/water solvent systems containing acids and contained magnesium and zinc. By comparison with authentic standards it was shown to be phytin, a salt of phytic acid, which is found in several commercially important oilseeds. The incomplete combustion is attributed to formation of oxides or carbonates of magnesium, calcium, or zinc. © 1979, Oxford University Press. All rights reserved.
引用
收藏
页码:514 / 517
页数:4
相关论文
共 5 条