A SIMPLE METHOD FOR CLONING GIARDIA-DUODENALIS FROM CULTURES AND FECAL SAMPLES

被引：20

作者：

BINZ, N ^{[1
]}

THOMPSON, RCA ^{[1
]}

MELONI, BP ^{[1
]}

LYMBERY, AJ ^{[1
]}

机构：

[1] DEPT AGR,S PERTH,WA 6151,AUSTRALIA

来源：

JOURNAL OF PARASITOLOGY | 1991年 / 77卷 / 04期

关键词：

D O I：

10.2307/3283171

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Using a novel method for cloning Giardia duodenalis from cultures and fecal samples, 47 clones from 7 isolates were established in vitro. Average colony-forming efficiency in established cultures was 43.2% compared to 11.2% when cloning directly from excystation. The highest success rate of cloning was found with the Portland (P1, ATCC No. 30888) isolate, with a colony-forming efficiency of 92.7%. Cloned and parent populations were compared over a range of 13 enzymes using starch gel electrophoresis. No genetic difference was found between any of the clones and the parent isolates.

引用

页码：627 / 631

页数：5

共 30 条

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