ENHANCED CHEMILUMINESCENT ENZYME-IMMUNOASSAY FOR THE DETECTION OF TRICHINOSIS ANTIBODIES IN PIGS

被引：5

作者：

KO, RC

YEUNG, MHF

机构：

[1] Department of Zoology, University of Hong Kong

来源：

VETERINARY PARASITOLOGY | 1992年 / 42卷 / 1-2期

关键词：

D O I：

10.1016/0304-4017(92)90106-J

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A modified enhanced chemiluminescent enzyme assay (ECIA) was developed for mass screening of pigs for trichinellosis antibodies in abattoirs. Using Bionectics beads as solid support, the assay time could be reduced to 45 min. Optimal conditions for washing, blocking, incubation, concentration of serum, antigens and conjugates as well as timing of film exposure were determined. The sensitivity and specificity of the assay were found to be comparable to those of the triple antibody-IgG ELISA. The assay was tested in an abattoir and its efficacy was found to be satisfactory. However, the major disadvantage of the assay is the high cost of magnetic beads.

引用

页码：101 / 110

页数：10

共 11 条

[1] COMPARISON BETWEEN STANDARD ELISA AND DOT-ELISA FOR SERODIAGNOSIS OF HUMAN TRICHINOSIS [J].