SCREENING BY POLYMERASE CHAIN-REACTION OF BACILLUS-THURINGIENSIS SEROTYPES FOR THE PRESENCE OF CRYV-LIKE INSECTICIDAL PROTEIN GENES AND CHARACTERIZATION OF A CRYV GENE CLONED FROM BACILLUS-THURINGIENSIS SUBSP KURSTAKI

被引：68

作者：

GLEAVE, AP

WILLIAMS, R

HEDGES, RJ

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 05期

关键词：

D O I：

10.1128/AEM.59.5.1683-1687.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polymerase chain reaction screening using cryV-specific oligonucleotides, designed to amplify the 5' half of cryV-type genes, revealed the presence of such genes in 7 of 21 Bacillus thuringiensis serotypes examined. Restriction analysis and hybridization studies indicated that these putative genes fall into at least three subclasses. The nucleotide sequence of the cryV-type gene cloned from B. thuringiensis subsp. kurstaki DSIR732 revealed an open reading frame coding for a protein of 719 amino acids, and lysates of Escherichia coli cells expressing the 81.2-kDa CryV732 protein were toxic to Epiphyas postvittana (Lepidoptera: Tortricidae).

引用

页码：1683 / 1687

页数：5

共 21 条

[1]

[Anonymous], 1971, PROBIT ANAL

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