PREIMPLANTATION EMBRYO DEVELOPMENT ENHANCED BY EPIDERMAL GROWTH-FACTOR

被引：9

作者：

BUYALOS, RP

CAI, XQ

机构：

[1] Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, UCLA School of Medicine, Los Angeles, 90024-1740, California, 10833 Le Conte Avenue

[2] Olympia Womens Health, Olympia, 98502, Washington

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1994年 / 11卷 / 01期

关键词：

EPIDERMAL GROWTH FACTOR; PREIMPLANTATION EMBRYO DEVELOPMENT; IN-VITRO FERTILIZATION;

D O I：

10.1007/BF02213695

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: Enhanced embryo maturity and advance stages of cleavage at the time of embryo transfer are associated with superior pregnancy rates in vitro fertilization procedures. This study was performed to assess the potential usefulness of epidermal growth factor (EGF) to enhance the development of murine preimplantation embryos cultured in vitro. Two-cell stage mouse embryos were cultured for 72 hr with EGF at concentrations of 0.1 to 100 ng/ml. The percentage of embryos which developed to the expanded and hatching blastocyst stage at 72 hr was determined. Results: The percentage of fully expanded and hatching murine blastocysts at 72 hr was significantly higher following incubation with EGF at concentrations of 2 ng/ml (44 +/- 4.1%; P < 0.02), 20 ng/ml (41 +/- 3.2%; P = 0.04),50 ng/ml (43 +/- 2.5%; P < 0.04), and 100 ng/ml (46 +/- 3.6%; P = 0.001) compared to controls. This effect of enhanced embryonic development by EGF was neutralized by co-incubation with 1.0 mug/ml of anti-EGF antibody. Conclusion: EGF at concentrations of 2 to 100 ng/ml significantly enhanced the percentage of expanded and hatching murine blastocysts at 72 hr.

引用

页码：33 / 37

页数：5

共 18 条

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