STANDARDIZED AGNOR STAINING METHOD FOR FORMALIN-FIXED AND PARAFFIN-EMBEDDED MATERIAL

被引：17

作者：

OFNER, D

BANKFALVI, A

RIEHEMANN, K

BOCKER, W

SCHMID, KW

机构：

[1] UNIV MUNSTER, GERHARD DOMAGK INST PATHOL, D-48149 MUNSTER, GERMANY

[2] UNIV INNSBRUCK, CHIRURG KLIN 1, INNSBRUCK, AUSTRIA

来源：

PATHOLOGE | 1994年 / 15卷 / 04期

关键词：

AGNOR STAINING METHOD; FORMALIN-FIXED MATERIAL; PARAFFIN-EMBEDDED MATERIAL;

D O I：

10.1007/s002920050049

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Visualization of proteins associated with nucleolar organizer regions proteins (AgNORs) on formalin-fixed and paraffin-embedded archival tissues is substantially improved after application of wet autoclave pretreatment. Silver staining results are comparable to those obtained on tissues processed in alcoholbased fixatives, illustrating AgNORs as substructures of the nucleoli without any staining artefacts. A highly reproducible staining quality was achieved irrespective of tissue origin or duration of formalin fixation. As a result of this novel and simple method, the grounds have been prepared for standardized AgNOR quantification on archival material.

引用

页码：226 / 230

页数：5

共 22 条

[1]

Aubele M., 1994, Zentralblatt fuer Pathologie, V140, P107

[2]

BANKFALVI A, IN PRESS J PATHOL

[3] HOW SHOULD WE COUNT AGNORS - PROPOSALS FOR A STANDARDIZED APPROACH [J].