EXPRESSION OF A FUNCTIONAL FUNGAL POLYKETIDE SYNTHASE IN THE BACTERIUM STREPTOMYCES-COELICOLOR A3(2)

被引：78

作者：

BEDFORD, DJ

SCHWEIZER, E

HOPWOOD, DA

KHOSLA, C

机构：

[1] STANFORD UNIV,DEPT CHEM ENGN,STANFORD,CA 94305

[2] UNIV ERLANGEN NURNBERG,LEHRSTUHL BIOCHEM,ERLANGEN,GERMANY

[3] JOHN INNES CTR,DEPT GENET,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 15期

关键词：

D O I：

10.1128/jb.177.15.4544-4548.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The multifunctional 6-methylsalicyiic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide pathways by heterologous expression of biosynthetic genes in an easily manipulated model bacterial host in which prokaryotic aromatic and modular polyketide synthase genes are already expressed and recombined.

引用

页码：4544 / 4548

页数：5