THE ROLE OF ETHYLENE IN SOMATIC EMBRYOGENESIS FROM LEAF DISCS OF COFFEA-CANEPHORA

被引：45

作者：

HATANAKA, T

SAWABE, E

AZUMA, T

UCHIDA, N

YASUDA, T

机构：

[1] KOBE UNIV, FAC AGR, KOBE, HYOGO 657, JAPAN

[2] KOBE UNIV, GRAD SCH SCI & TECHNOL, KOBE, HYOGO 657, JAPAN

来源：

PLANT SCIENCE | 1995年 / 107卷 / 02期

关键词：

COBALT CHLORIDE; COFFEE (COFFEA CANEPHORA); ETHYLENE; SILVER NITRATE; SOMATIC EMBRYOGENESIS;

D O I：

10.1016/0168-9452(95)04103-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of ethylene in the formation of somatic embryos from leaf discs of Coffea canephora was studied on a medium that contained a cytokinin, iso-pentenyladenine, as the sole plant growth regulator. During incubation under these conditions, explants always produced a small amount of ethylene. Removal of this ethylene by an absorbent reduced the number of somatic embryos induced by the cytokinin, Application of inhibitors of the production of ethylene (Co2+ ions) and of the action of ethylene (Ag+ ions) inhibited the formation of embryos. Exogenous ethylene gas (12 mu l/l) partially overcame the effect of Co2+ ions. These results indicate that ethylene plays an important role in regulating somatic embryogenesis in leaf cultures of Coffea canephora.

引用

页码：199 / 204

页数：6

共 17 条

[1]

AARTRIJK J, 1985, J PLANT PHYSL, V117, P401

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