ENDOGENOUS, CYCLIC 3'-5' AMP-DEPENDENT PHOSPHORYLATION OF HUMAN RED-CELL PYRUVATE-KINASE

被引：28

作者：

MARIE, J ^{[1
]}

TICHONICKY, L ^{[1
]}

DREYFUS, JC ^{[1
]}

KAHN, A ^{[1
]}

机构：

[1] INST PATHOL MOLEC,24 RUE FAUBOURG ST JACQUES,F-75674 PARIS 14,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)92037-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP-depleted human red cells have been incubated in a glucosecontaining medium with ortho [32P] phosphate in the presence and in the absence of cyclic AMP and dibutyril cyclic AMP. Then pyruvate kinase has been purified to homogeneity. It was found that pyruvate kinase was intensively phosphorylated in the presence of cyclic 3′-5′ AMP and 5 times less in its absence. The phosphorylated site was readily cleaved by subtilisin. © 1979.

引用

页码：862 / 868

页数：7

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