RAT TRACHEAL EPITHELIAL-CELLS PRODUCE GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR

It is unknown whether local resident cells of the upper airway are able to regulate the number and function of phagocytic cells by the secretion of cytokines. We undertook to determine if tracheal epithelial cells (TEC) produce the potent cytokine granulocyte/macrophage colony-stimulating factor (GM-CSF) and how TEC-derived GM-CSF might be regulated. Conditioned media (TEC-CM) from 7- to 21-day-old primary cultures of rat TEC contained material with bioactivity similar to GM-CSF. This bioactivity was increased in conditioned medium from lipopolysaccharide (LPS)-treated (1 microgram/ml) TEC. Molecular characterization of bioactivity revealed a molecular weight of 27 to 44 kD by gel-filtration high performance liquid chromatography (HPLC), and elution at 44 to 50% acetonitrile by reverse-phase HPLC, similar to that of authentic GM-CSF. The biologic activity of TEC-CM was completely blocked by a goat polyclonal anti-GM-CSF antibody. With in situ hybridization using a murine GM-CSF cDNA probe, more than 95% of the adherent TEC population expressed GM-CSF transcripts, and the number of transcripts was significantly increased by LPS (1 microgram/ml, 48 h). TEC appear to produce a cytokine that is functionally, biochemically, and antigenically indistinguishable from GM-CSF. The ability of TEC to produce GM-CSF suggests that these cells may play a role in modulating the inflammatory response in the airway.