LIPOSOME-MEDIATED DNA UPTAKE BY SPERM CELLS

被引：85

作者：

BACHILLER, D

SCHELLANDER, K

PELI, J

RUTHER, U

机构：

[1] EUROPEAN MOLEC BIOL LAB,POB 102209,W-6900 HEIDELBERG,GERMANY

[2] VET UNIV VIENNA,INST TIERZUCHT & GENET,A-1030 VIENNA,AUSTRIA

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 1991年 / 30卷 / 03期

关键词：

DNA TRANSFER; TRANSGENIC ANIMALS; CATIONIC LIPOSOMES;

D O I：

10.1002/mrd.1080300305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the potential use of sperm cells as vectors to transfer exogenous DNA via the fertilization of oocytes into the germ line of mice, we have used liposomes to transfect DNA into the sperm head. Although the DNA transfer into sperm mediated by liposomes was very efficient and no obvious reduction in the fertilization frequency of oocytes could be detected, we were unable to generate transgenic mice by this method.

引用

页码：194 / 200

页数：7

共 9 条

[1]

[Anonymous], 1986, MANIPULATING MOUSE E

[2] EFFICIENT GENE-TRANSFER INTO MAMMALIAN PRIMARY ENDOCRINE-CELLS WITH LIPOPOLYAMINE-COATED DNA [J].