SUBSTITUTION OF PHOSPHOTYROSINE FOR SULPHOTYROSINE IN BIOLOGICALLY-ACTIVE PEPTIDES - ENZYMATIC PHOSPHORYLATION OF A PROGASTRIN PEPTIDE CONFERS IMMUNOREACTIVITY REMINISCENT OF THE SULFATED DERIVATIVE

被引:7
作者
DONELLADEANA, A
VARRO, A
DOCKRAY, GJ
PINNA, LA
机构
[1] UNIV PADUA,DIPARTIMENTO CHIM BIOL,VIA TRIESTE 75,I-35121 PADUA,ITALY
[2] CTR STUDIO FISIOL MITOCONDRIALE,PADUA,ITALY
[3] UNIV LIVERPOOL,DEPT PHYSIOL,LIVERPOOL L69 3BX,ENGLAND
关键词
TYROSINE PROTEIN KINASE; PROGASTRIN; PHOSPHOTYROSINE; SULFOTYROSINE; TYROSINE PROTEIN KINASE SPECIFICITY; (RAT);
D O I
10.1016/0167-4889(91)90046-Z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The peptide SAEEEDQYN, corresponding to the carboxyl-terminal tryptic fragment of rat progastrin, whose penultimate tyrosyl residue is sulphated in the native peptide, is phosphorylated with K(m) values of 120 and 180-mu-M by two spleen tyrosine protein kinases, termed TPK-IIB and TPK-III, respectively. Another spleen tyrosine protein kinase related to the src family (TPK-I/lyn) is poorly active toward this peptide, displaying a K(m) 6.5 mM. The Tyr-phosphorylated peptide is recognized by an antibody (L304), which reacts with both sulphated and unmodified peptides, while it is not recognized by a second antibody (L303), which reacts with unmodified peptide yet not with the sulphated derivative. These data, in conjunction with previous observations (Hofsteenge, J., Stone, S.R., Donella-Deana, A. and Pinna, L.A. (1990) Eur. J. Biochem. 188, 55-59) support the view that phosphotyrosine is an effective surrogate for sulphotyrosine in a wide spectrum of biological activities.
引用
收藏
页码:75 / 77
页数:3
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