NANOSCALE SEPARATIONS - CAPILLARY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY FOR THE DETERMINATION OF PEPTIDES AND PROTEINS

被引：50

作者：

DETERDING, LJ

PARKER, CE

PERKINS, JR

MOSELEY, MA

JORGENSON, JW

TOMER, KB

机构：

[1] NIEHS, MOLEC BIOPHYS LAB, POB 12233, RES TRIANGLE PK, NC 27709 USA

[2] UNIV N CAROLINA, DEPT CHEM, CHAPEL HILL, NC 27514 USA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 554卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88460-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nanoscale separation techniques, such as packed capillary liquid chromatography and capillary zone electrophoresis in which the column effluent flow-rates are in the nanoliter per minute range rather than microliter per minute range, have been successfully interfaced with continuous-flow fast atom bombardment and electrospray ionization. Applications of these techniques to the separation and determination of peptide mixtures, protein digests and protein mixtures are presented.

引用

页码：329 / 338

页数：10

共 22 条

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