MONOCLONAL-ANTIBODY IMMUNOASSAY FOR QUANTITATIVE-ANALYSIS OF GROUP-V ALLERGENS IN GRASS-POLLEN EXTRACTS

被引：12

作者：

FAHLBUSCH, B ^{[1
]}

MULLER, WD ^{[1
]}

SCHLENVOIGT, G ^{[1
]}

JAGER, L ^{[1
]}

WAHL, R ^{[1
]}

WEBER, B ^{[1
]}

机构：

[1] JOACHIM GANZER KG, ALLERGOPHARMA, REINBEK, GERMANY

来源：

CLINICAL AND EXPERIMENTAL ALLERGY | 1993年 / 23卷 / 09期

关键词：

D O I：

10.1111/j.1365-2222.1993.tb00362.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

A two-site monoclonal antibody (MoAb) ELISA has been developed for the quantification of the Phleum pratense major allergen, Phl p V. The assay is based on two MoAbs which recognize different non-overlapping epitopes on the Phl p V molecule; one antibody (1D11) was immobilized on the solid phase and the other (3B2) was biotinylated. An affinity-purified Phl p V preparation (purity of 95%) was used as standard. The assay has a sensitivity of 10 ng/ml of allergen and is suitable for the detection of group V allergen in aqueous grass extracts. The specificity of the assay was investigated with 14 grass pollen and five non-grass pollen extracts. Different levels of group V allergen were detected in extracts of grasses, but not in non-grasses. The assay gives a good correspondence with allergenic activity of extracts as determined by ELISA inhibition using serum pool of allergic patients. The results indicate that the two-site MoAb ELISA could be very useful in the standardization of allergenic extracts from grass pollen.

引用

页码：747 / 754

页数：8

共 25 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] MONOCLONAL IMMUNOASSAYS FOR MAJOR DUST MITE (DERMATOPHAGOIDES) ALLERGENS, DER-P-I AND DER-F-I, AND QUANTITATIVE-ANALYSIS OF THE ALLERGEN CONTENT OF MITE AND HOUSE DUST EXTRACTS [J].