HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION AND ELECTROCHEMICAL DETECTION OF PENICILLINS

被引：25

作者：

KIRCHMANN, E ^{[1
]}

WELCH, LE ^{[1
]}

机构：

[1] KNOX COLL, DEPT MED, GALESBURG, IL 61401 USA

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 633卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)83144-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatography method used to determine penicillins is described. A C18 stationary phase is used in conjunction with mixed solvent systems containing acetate buffer, methanol, and acetonitrile in various proportions. An isocratic separation is illustrated, but usage of a gradient program varying the composition of the organic modifier gives better performance. Detection is accomplished using pulsed amperometric detection to indirectly monitor the penicillins. The detection limit for ampicillin is 4 . 10(-7) M.

引用

页码：111 / 118

页数：8

共 38 条

[1]

BESADA A, 1987, MIKROCHIM ACTA, V2, P225

[2] HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PENICILLIN-G POTASSIUM AND ITS DEGRADATION PRODUCTS [J].