A HEMI-NESTED PCR ASSAY FOR THE DETECTION AND IDENTIFICATION OF VESICULAR STOMATITIS-VIRUS NUCLEIC-ACID

被引：13

作者：

HOFNER, MC ^{[1
]}

CARPENTER, WC ^{[1
]}

FERRIS, NP ^{[1
]}

KITCHING, RP ^{[1
]}

BOTERO, FA ^{[1
]}

机构：

[1] ICA,CEISA,SANTAFE DE BOGATA,COLOMBIA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1994年 / 50卷 / 1-3期

关键词：

POLYMERASE CHAIN REACTION; HEMI-NESTED PCR; VESICULAR STOMATITIS VIRUS;

D O I：

10.1016/0166-0934(94)90159-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper is the first to describe the development of a hemi-nested PCR assay for the detection of vesicular stomatitis virus (VSV) nucleic acid. This assay was developed as it combines high sensitivity for virus genome detection with the identification of the external amplification product in the reamplification step, thus confirming the specificity of the reaction. The assay did not depend on the presence of infectious virus in samples, as demonstrated by its detection of VSV in blood samples which were non-infectious in tissue culture. One further advantage was that the VSV-New Jersey and VSV-Indiana serotypes could be differentiated through the selective use of the appropriate hemi-nested primer. This assay is ideal for the study of VSV pathogenesis and persistence.

引用

页码：11 / 20

页数：10

共 23 条

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