COMPETITIVE ADSORPTION OF HIGH-MOLECULAR-WEIGHT KININOGEN AND FIBRINOGEN FROM BINARY-MIXTURES TO GLASS-SURFACE

被引:32
作者
DEJARDIN, P
TENHOVE, P
YU, XJ
BRASH, JL
机构
[1] MCMASTER UNIV, DEPT CHEM ENGN, HAMILTON, ON L8S 4L8, CANADA
[2] INST CHARLES SADRON, F-67083 STRASBOURG, FRANCE
关键词
D O I
10.1021/la00010a061
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An investigation of adsorption from binary mixtures of fibrinogen and high molecular weight kininogen (KK) to glass is reported. Experiments were performed using radioiodinated proteins in which the adsorption of each protein was measured from serial dilutions of mixtures having a ratio of the proteins approximately the same as in plasma (fibrinogen in excess). Fibrinogen adsorption passes through a maximum as solution concentration increases, analogous to its behavior in plasma as reported previously (Brash, J. L.; ten Hove, P. Thromb. Haemostasis, 1984, 51, 326). HK adsorption increases monotonically with concentration such that fibrinogen is virtually excluded from the surface at the highest concentrations even though present in large excess. A kinetic model is developed in which initially adsorbed fibrinogen can either desorb spontaneously, relax to an irreversibly bound state, or exchange with HK. The model solution is obtained in terms of the ratio of the rate constants for exchange and relaxation, k(ex)/k(1)(') and is independent of the nature of the available surface function. Data fits to the model are excellent and give essentially invariant values of k(ex)/k(1)(') for different ratios of the two proteins in solution. The results of this study emphasize the importance of surface relaxation in competitive protein adsorption.
引用
收藏
页码:4001 / 4007
页数:7
相关论文
共 30 条
[1]   CONVEX-LENS-ON-SLIDE - A SIMPLE SYSTEM FOR THE STUDY OF HUMAN-PLASMA AND BLOOD IN NARROW SPACES [J].
ADAMS, AL ;
FISCHER, GC ;
MUNOZ, PC ;
VROMAN, L .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1984, 18 (06) :643-654
[2]   INHIBITION OF CELL-ADHESION BY HIGH-MOLECULAR-WEIGHT KININOGEN [J].
ASAKURA, S ;
HURLEY, RW ;
SKORSTENGAARD, K ;
OHKUBO, I ;
MOSHER, DF .
JOURNAL OF CELL BIOLOGY, 1992, 116 (02) :465-476
[3]  
BRASH JL, 1984, THROMB HAEMOSTASIS, V51, P326
[4]  
BRASH JL, 1988, BLOOD, V71, P932
[5]   PREFERENTIAL ADSORPTION OF HIGH-DENSITY LIPOPROTEIN FROM BLOOD-PLASMA ONTO BIOMATERIAL SURFACES [J].
BREEMHAAR, W ;
BRINKMAN, E ;
ELLENS, DJ ;
BEUGELING, T ;
BANTJES, A .
BIOMATERIALS, 1984, 5 (05) :269-274
[6]   ADSORPTION OF FIBRINOGEN ON GLASS - REVERSIBILITY ASPECTS [J].
CHAN, BMC ;
BRASH, JL .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1981, 82 (01) :217-225
[7]   A PRELIMINARY COMPARISON OF THE THROMBOGENIC ACTIVITY OF VITRONECTIN AND OTHER RGD-CONTAINING PROTEINS WHEN BOUND TO SURFACES [J].
COLLINS, WE ;
MOSHER, DF ;
TOMASINI, BR ;
COOPER, SL .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1987, 516 :291-299
[8]   MEASUREMENT OF PROTEIN ADSORPTION-KINETICS BY AN INSITU, REAL-TIME, SOLUTION DEPLETION TECHNIQUE [J].
CORNELIUS, RM ;
WOJCIECHOWSKI, PW ;
BRASH, JL .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1992, 150 (01) :121-133
[9]   ADSORPTION-KINETICS OF PROTEIN MIXTURES - A TENTATIVE EXPLANATION OF THE VROMAN EFFECT [J].
CUYPERS, PA ;
WILLEMS, GM ;
HEMKER, HC ;
HERMENS, WT .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1987, 516 :244-252
[10]   RATIO OF FINAL INTERFACIAL CONCENTRATIONS IN EXCHANGE PROCESSES [J].
DEJARDIN, P ;
LE, MT .
LANGMUIR, 1995, 11 (10) :4008-4012