Quantitative and structural analysis of inhibitors of phospholipase A(2)

被引：13

作者：

Gelb, Michael H. ^{[1
]}

Berg, Otto ^{[2
]}

Jain, Mahendra K. ^{[3
]}

机构：

[1] Univ Washington, Dept Chem, BG-10, Seattle, WA 98195 USA

[2] Uppsala Univ, Dept Mol Biol, Uppsala, Sweden

[3] Univ Delaware, Dept Chem & Biochem, Newark, DE 19716 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1991年 / 1卷 / 05期

关键词：

D O I：

10.1016/0959-440X(91)90187-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The evaluation of competitive inhibitors of phospholipase A(2) is best carried out by analysing the enzyme under conditions in which it binds irreversibly to the substrate vesicle and catalyses a processive reaction in which all of the phospholipid molecules on the outer layer of the vesicle are hydrolysed. Recent structural analyses of phospholipase A(2)-inhibitor complexes suggest a mechanism for the catalytic site.

引用

页码：836 / 843

页数：8

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