MEMBRANE PARTITIONING OF ROSE-TYPE CHAMBER FOR STUDY OF METABOLIC INTERACTION BETWEEN DIFFERENT CULTURES

被引:3
作者
BATZDORF, U
KNOX, RS
POKRESS, SM
KENNADY, JC
机构
[1] Department of Surgery/Neurosurgery, UCLA School of Medicine, The Brain Research Institute, Los Angeles, CA
来源
STAIN TECHNOLOGY | 1969年 / 44卷 / 02期
关键词
D O I
10.3109/10520296909063327
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Rose tissue culture chamber has been divided into 2 compartments by using half-thickness gaskets with an interposed Nuclepore (General Electric Co.) filter. This permits study of the interaction of 2 different cell populations on each side of the membrane. Radioiodinated serum albumin concentration studies demonstrate rapid distribution of labelled protein across the membrane while its pore size of 0.5 μ or 0.8 μ prevents migration of the cells themselves. Equilibrium between plain medium and radioactive medium was established in 7-9 hr. © 1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码:71 / &
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