INVITRO EVALUATION OF BIOCOMPATIBILITY OF SURFACE-MODIFIED POLY(METHYL METHACRYLATE) PLATE WITH RABBIT LENS EPITHELIAL-CELLS

被引：6

作者：

ICHIJIMA, H ^{[1
]}

KOBAYASHI, H ^{[1
]}

IKADA, Y ^{[1
]}

机构：

[1] KYOTO UNIV,BIOMED ENGN RES CTR,53 KAWAHARACHO,SAKYO KU,KYOTO 606,JAPAN

来源：

JOURNAL OF CATARACT AND REFRACTIVE SURGERY | 1992年 / 18卷 / 04期

关键词：

COLLAGEN TYPE-I; MOSAIC CELL APPEARANCE; POLY(METHYL METHACRYLATE); PSEUDOPODIA; RABBIT LENS EPITHELIAL CELL; SURFACE MODIFICATION;

D O I：

10.1016/S0886-3350(13)80079-X

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Collagen type I was immobilized onto a poly(methyl methacrylate) (PMMA) plate by covalent bonding following surface modification by two methods. One method introduced amino groups by aminolysis with N-lithioethylenediamine (PMMA-NH2) and the other introduced carboxyl groups by graft copolymerization of acrylic acid (AAc) and acrylamide (AAm) (PMMA-COOH). Lens epithelial rabbit cells were cultured on the PMMA plate which was immobilized with collagen. Polygonal cells with a mosaic appearance were observed on the PMMA-COOH plate immobilized with collagen type I, whereas pleomorphic cells were present on the virgin PMMA and on the PMMA-NH2 plate immobilized with collagen type I. We concluded the PMMA-COOH plate immobilized with collagen type I provided a more comfortable atmosphere for lens epithelial cells, causing no metaplasia, than the other plates used in this cell culture model experiment.

引用

页码：395 / 401

页数：7

共 21 条

[1]

Lindstrom RL, Harris WS, Management of the posterior capsule following posterior chamber lens implantation, Am Intra-Ocular Implant Soc J, 6, pp. 255-258, (1980)

[2]

Nishi O, Removal of lens epithelial cells by ultrasound in endocapsular cataract surgery, Ophthalmic Surg, 18, pp. 577-580, (1987)

[3]

Wolter JR, Felt DP, Proliferation of fibroblast-like cells on failing intraocular lenses, Ophthalmic Surg, 14, pp. 57-64, (1983)

[4]

Sievers H, von Domarus D, Foreign-body reaction against intraocular lenses, Am J Ophthalmol, 97, pp. 743-751, (1984)

[5]

Greenburg G, Hay ED, Cytodifferentiation and tissue phenotype change during transformation of embryonic lens epithelium to mesenchyme-like cells in vitro, Dev Biol, 115, pp. 363-379, (1986)

[6]

Santos BA, DelMonte MA, Pastora R, O'Donnell FE, Lens epithelial inhibition by PMMA optic: Implications for lens design, J Cataract Refract Surg, 12, pp. 23-26, (1986)

[7]

Sterling S, Wood TO, Effect of intraocular lens convexity on posterior capsule opacification, J Cataract Refract Surg, 12, pp. 655-657, (1986)

[8]

Hansen TE, Otland N, Corydon L, Posterior capsule fibrosis and intraocular lens design, J Cataract Refract Surg, 14, pp. 383-386, (1988)

[9]

Fagerholm P, Bjorklund H, Holmberg A, Et al., Heparin surface modified intraocular lenses implanted in the monkey eye, J Cataract Refract Surg, 15, pp. 485-490, (1989)

[10]

Balyeat HD, Nordquist RE, Lerner MP, Gupta A, Comparison of endothelial damage produced by control and surface modified poly(methyl methacrylate) intraocular lenses, J Cataract Refract Surg, 15, pp. 491-494, (1989)

← 1 2 3 →