COMPARATIVE-ANALYSIS OF THE METHYLATION STATUS OF THE 5' FLANKING REGION OF HA-RAS IN B6C3F1, C3H/HE AND C57BL/6 MOUSE-LIVER

被引：9

作者：

COUNTS, JL ^{[1
]}

GOODMAN, JI ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,INST ENVIRONM TOXICOL,DEPT PHARMACOL & TOXICOL,B440 LIFE SCI BLDG,E LANSING,MI 48824

来源：

CANCER LETTERS | 1993年 / 75卷 / 02期

关键词：

MOUSE LIVER TUMOR; HA-RAS; METHYLATION; DNA; 5-METHYLCYTOSINE; ONCOGENE;

D O I：

10.1016/0304-3835(93)90197-H

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We examined the methylation status of the 5' flanking region of Ha-ras in the liver of the liver tumor-prone B6C3F1 male (C57BL/6 female x C3H/He male) and C3H/He male, plus the relatively resistant C57BL/6 male mouse strains. Southern analysis revealed the presence of CCGG sites methylated at the internal cytosine, as well as unmethylated CCGG sites in all three strain's. Digestion with StyI and XhoI revealed an unmethylated Xhol site in the C57BL/6 male. This pattern is not obvious in the B6C3F1 or C3H/He, indicating sequence variation and/or less methylation of Ha-ras in those strains of mice that exhibit a high propensity towards development of liver tumors.

引用

页码：129 / 136

页数：8

共 34 条

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