The acute and chronic toxicity of nonylphenol to the freshwater invertebrate Daphnia magna has been determined. The acute 24 and 48 h EC50 values, based on immobilization, were 0.30 and 0.19 mg/l, respectively, using mean measured concentrations. In a separate study, effects of nonylphenol on survival, growth and reproduction of D. magna were measured over a period of 21 days. The no observed effect concentration (NOEC), based on mean measured concentrations, was found to be 0.024 mg/l. This is approximately one order of magnitude below the acute value and one order of magnitude higher than the highest reported environmental levels.