How to detect Toxoplasma gondii oocysts in environmental samples?

被引:165
作者
Dumetre, A [1 ]
Darde, ML [1 ]
机构
[1] Fac Med Limoges, UPRES EQA 3174, F-87025 Limoges, France
关键词
Toxoplasma gondii; oocyst; felid; water; environment; detection;
D O I
10.1016/S0168-6445(03)00071-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Detection of Toxoplasma gondii oocysts in environmental samples is a great challenge for researchers as this coccidian parasite can be responsible for severe infections in humans and in animals via ingestion of a single oocyst from contaminated water, soil, fruits or vegetables. Despite field investigations, oocysts have been rarely recovered from the environment due to the lack of sensitive methods. Immunomagnetic separation, fluorescence-activated cell sorting, and polymerase chain reaction have recently shown promising use in detection of protozoa from complex matrices. Such procedures could be applied to T gondii detection, if studies on the antigenic and biochemical composition of the oocyst wall are completed. Using such methods, it will be possible to assess the occurrence, prevalence, viability and virulence of T gondii oocysts in environmental matrices and specify sources of human and animal contamination. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:651 / 661
页数:11
相关论文
共 108 条
[1]   Microsatellite analysis of Toxoplasma gondii shows considerable polymorphism structured into two main clonal groups [J].
Ajzenberg, D ;
Bañuls, AL ;
Tibayrenc, M ;
Dardé, ML .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2002, 32 (01) :27-38
[2]   RECOVERY OF CRYPTOSPORIDIUM OOCYSTS FROM WATER BY A MEMBRANE-FILTER DISSOLUTION METHOD [J].
ALDOM, JE ;
CHAGLA, AH .
LETTERS IN APPLIED MICROBIOLOGY, 1995, 20 (03) :186-187
[3]   Potential contamination of drinking water with Toxoplasma gondii oocysts [J].
Aramini, JJ ;
Stephen, C ;
Dubey, JP ;
Engelstoft, C ;
Schwantje, H ;
Ribble, CS .
EPIDEMIOLOGY AND INFECTION, 1999, 122 (02) :305-315
[4]   Toxoplasma gondii in Vancouver Island cougars (Felis concolor vancouverensis):: Serology and oocyst shedding [J].
Aramini, JJ ;
Stephen, C ;
Dubey, JP .
JOURNAL OF PARASITOLOGY, 1998, 84 (02) :438-440
[5]  
Bahia-Oliveira LMG, 2003, EMERG INFECT DIS, V9, P55
[6]   OOCYST-TRANSMITTED TOXOPLASMOSIS ASSOCIATED WITH INGESTION OF CONTAMINATED WATER [J].
BENENSON, MW ;
TAKAFUJI, ET ;
LEMON, SM ;
GREENUP, RL ;
SULZER, AJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1982, 307 (11) :666-669
[7]   Autofluorescence and the detection of Cyclospora oocysts [J].
Berlin, OGW ;
Peter, JB ;
Gagne, C ;
Conteas, CN ;
Ash, LR .
EMERGING INFECTIOUS DISEASES, 1998, 4 (01) :127-128
[8]   Population biology of Toxoplasma gondii and its relevance to human infection:: do different strains cause different disease? [J].
Boothroyd, JC ;
Grigg, ME .
CURRENT OPINION IN MICROBIOLOGY, 2002, 5 (04) :438-442
[9]   Outbreak of toxoplasmosis associated with municipal drinking water [J].
Bowie, WR ;
King, AS ;
Werker, DH ;
IsaacRenton, JL ;
Bell, A ;
Eng, SB ;
Marion, SA .
LANCET, 1997, 350 (9072) :173-177
[10]  
Bukhari Z, 1998, APPL ENVIRON MICROB, V64, P4495