FUNCTIONAL-ANALYSIS OF DNA-ELEMENTS INVOLVED IN TRANSCRIPTIONAL CONTROL OF THE HUMAN GLUCOSE-TRANSPORTER-2 (GLUT-2) GENE IN THE INSULIN-PRODUCING CELL-LINE-BETA-TC-3

被引:8
作者
LEIBIGER, B [1 ]
LEIBIGER, IB [1 ]
机构
[1] UNIV GREIFSWALD,SCH MED,INST BIOCHEM,GREIFSWALD,GERMANY
关键词
GENE EXPRESSION REGULATION; INSULINOMA; GLUCOSE TRANSPORTER; TRANSCRIPTION; PROMOTER;
D O I
10.1007/BF02369360
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The uptake of glucose into pancreatic beta cells as a 'non-rate-limiting-step' is guaranteed by the expression and action of the high-K-m glucose transporter 2 (GLUT 2). This transporter is not saturated by physiological plasma glucose levels and hence functions as a ''glucose sensor/glucoreceptor''. Here we describe DNA-elements of the human GLUT 2 gene promoter which contribute to transcriptional control in the insulin-producing cell line (beta TC-3. Nested 5'- as well as 3'-deletions of a DNA-fragment containing up to 1245 bp of the 5'-flanking region and up to 308 bp of the first exon of the human GLUT 2 gene were investigated for their ability to control the expression of a CAT reporter gene in beta TC-3 cells. For tissue-specific transcriptional control 5'-deletional analysis revealed that the region -220/+309 was sufficient. Truncation from the 3'-end from nucleotide +308 to +204 led to a threefold drop in CAT expression. In vitro DNase I footprinting analysis was performed to delineate cis-elements within the region -220/+1. Five specifically protected areas could be defined.
引用
收藏
页码:112 / 115
页数:4
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