ESTIMATION OF ADENINE NUCLEOTIDES IN BRAIN BY ENZYMIC AND ION-EXCHANGE CHROMATOGRAPHIC METHODS

被引：6

作者：

WILSON, WS

THOMSON, RY

机构：

[1] Departments of Pharmacology and Biochemistry, Glasgow University, Glasgow, Scotland

来源：

BIOCHEMICAL PHARMACOLOGY | 1969年 / 18卷 / 06期

关键词：

D O I：

10.1016/0006-2952(69)90242-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A spectrophotometric assay for the adenine nucleotides using AMP deaminase, myokinase (MK) and apyrase has been examined and found to give erroneously high estimates of AMP, ADP and ATP in rat brain extract. The AMP deaminase preparation used is liable to contain creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) activities. However, it is suggested that the discrepancy may be due to the presence of yet another enzymic contaminant. Some aspects of nucleotide extraction from brain tissue are investigated. © 1969.

引用

页码：1297 / +

页数：1

共 25 条

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