CHARACTERIZATION OF INTERFERON MESSENGER-RNA FROM HUMAN-LYMPHOBLASTOID CELLS

被引：31

作者：

MORSER, J

FLINT, J

MEAGER, A

GRAVES, H

BAKER, PN

COLMAN, A

BURKE, DC

机构：

来源：

JOURNAL OF GENERAL VIROLOGY | 1979年 / 44卷 / JUL期

关键词：

D O I：

10.1099/0022-1317-44-1-231

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

After treatment with Sendai virus, Namalwa cells form large amounts of interferon. RNA extracted from treated whole cells or from their polysomes was injected into Xenopus laevis oocytes and the interferon formed was titrated. The results show that the amount of interferon mRNA was maximal by 9 h after treatment of the cells with Sendai virus and then declined. Sucrose gradient centrifugation of the mRNA gave substantial purification and showed that its size was 12 S.

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页码：231 / 234

页数：4

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