PERDEUTERIOACETYLATION WITH COMBINED NMR AND MS ANALYSIS AS A METHOD FOR DETERMINING THE EVOLUTION OF INDIVIDUAL HYDROLYSIS PRODUCTS DURING REGIOSELECTIVE ENZYMATIC-HYDROLYSIS OF GLUCOSE PENTAACETATE

被引：4

作者：

CHAPLIN, D ^{[1
]}

CROUT, DHG ^{[1
]}

HUTCHINSON, DW ^{[1
]}

HOWARTH, OW ^{[1
]}

KHAN, R ^{[1
]}

机构：

[1] TATE & LYLE SPECIAL SWEETENERS,READING RG6 2BX,ENGLAND

来源：

CATALYSIS LETTERS | 1991年 / 9卷 / 1-2期

关键词：

LIPASE; HYDROLYSIS; BETA-D-GLUCOSE PENTAACETATE; PERDEUTERIOACETYLATION;

D O I：

10.1007/BF00769084

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A new procedure is described that is based on NMR and MS analysis of the products of perdeuterioacetylation of the products of partial hydrolysis of beta-D-glucose pentaacetate by the lipase from Aspergillus niger. This procedure eliminates the need for separation and estimation of each individual product and is of general applicability.

引用

页码：71 / 84

页数：14

共 19 条

[1] ENZYME CATALYZED PREPARATION OF 6-O-ACYLGLUCOPYRANOSIDES [J].

ADELHORST, K ;

BJORKLING, F ;

GODTFREDSEN, SE ;

KIRK, O .

SYNTHESIS-STUTTGART, 1990, (02) :112-115

[2] REGIOSELECTIVE DEACYLATION OF 1,6-ANHYDRO-BETA-D-GALACTOPYRANOSE DERIVATIVES CATALYZED BY SOLUBLE AND IMMOBILIZED LIPASES [J].

BALLESTEROS, A ;

BERNABE, M ;

CRUZADO, C ;

MARTINLOMAS, M ;

OTERO, C .

TETRAHEDRON, 1989, 45 (22) :7077-7082

[3] AN IMPROVED METHOD FOR HETERONUCLEAR CHEMICAL-SHIFT CORRELATION BY TWO-DIMENSIONAL NMR [J].

BAX, A ;

MORRIS, GA .

JOURNAL OF MAGNETIC RESONANCE, 1981, 42 (03) :501-505

[4] A HIGHLY SELECTIVE ENZYME-CATALYZED ESTERIFICATION OF SIMPLE GLUCOSIDES [J].

BJORKLING, F ;

GODTFREDSEN, SE ;

KIRK, O .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1989, (14) :934-935

[5] ENZYMATIC-SYNTHESIS OF VARIOUS 1'-O-SUCROSE AND 1-O-FRUCTOSE ESTERS [J].

CARREA, G ;

RIVA, S ;

SECUNDO, F ;

DANIELI, B .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1989, (05) :1057-1061

[6]

HONIG H, 1989, TETRAHEDRON, V330, P811

[7] APPLICATION OF LIPASES IN THE REMOVAL OF PROTECTIVE GROUPS ON GLYCERIDES AND GLYCOSIDES [J].

KLOOSTERMAN, M ;

MOSMULLER, EWJ ;

SCHOEMAKER, HE ;

MEIJER, EM .

TETRAHEDRON LETTERS, 1987, 28 (26) :2989-2992

[8] REGIOSELECTIVE HYDROLYSIS OF CARBOHYDRATE SECONDARY ACYL ESTERS BY LIPASES [J].

KLOOSTERMAN, M ;

DENIJS, MP ;

WEIJNEN, JGJ ;

SCHOEMAKER, HE ;

MEIJER, EM .

JOURNAL OF CARBOHYDRATE CHEMISTRY, 1989, 8 (03) :333-341

[9] AN INTERESTING EXAMPLE OF COMPLEMENTARY REGIOSELECTIVE ACYLATION OF SECONDARY HYDROXYL-GROUPS BY DIFFERENT LIPASES [J].

NICOTRA, F ;

RIVA, S ;

SECUNDO, F ;

ZUCCHELLI, L .

TETRAHEDRON LETTERS, 1989, 30 (13) :1703-1704

[10] CONCERTED USE OF HOMO-NUCLEAR AND HETERO-NUCLEAR 2D NMR - C-13 AND H-1 ASSIGNMENT OF SUCROSE OCTAACETATE [J].

NISHIDA, T ;

ENZELL, CR ;

MORRIS, GA .

MAGNETIC RESONANCE IN CHEMISTRY, 1986, 24 (02) :179-182

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