VENTILATORY AND METABOLIC RESPONSES TO COLD AND HYPOXIA IN INTACT AND CAROTID BODY-DENERVATED RATS

The effects of hypoxia on thermoregulation and ventilatory control were studied in conscious rats before and after carotid denervation (CD). Measurements of metabolic rate (VO2), ventilation (V), shivering intensity (SI), and colonic temperature (T(c)) were made in groups of eight rats subjected to three protocols. In protocols 1 and 2, at ambient temperature (T(a)) of 25 and 5-degrees-C, respectively, rats were exposed to normoxia and hypoxia [inspired O2 fraction (FI(O2)) 0.13-0.11]. In protocol 3, T(a) was decreased from 25 to 5-degrees-C in 30-min steps of 5-degrees-C. Recordings were made in normoxia and hypoxia (FI(O2) 0.12). The results show that in both intact and CD rats 1) in normoxia, cold exposure increased VO2, V, and SI, and these increases were proportional to the decrease in T(a); 2) hypoxia induced only a transient decrease in SI, and, for a given T(a), VO2 was reduced whereas V and SI were increased; and 3) in CD rats, V increased less during cold exposure in both normoxia and hypoxia; VO2 and T(c) were more depressed during hypoxia. It is concluded that 1) the interaction between T(a) and FI(O2) in the control of V is partly dependent on the carotid body afferents, 2) shivering thermogenesis may be transiently affected by hypoxia independently of the carotid body afferents, and 3) nonshivering thermogenesis may be directly inhibited by hypoxia, especially during cold exposure.