LEUKEMIA INHIBITORY FACTOR (LIF) MEDIATED INCREASE OF CHOLINE-ACETYLTRANSFERASE ACTIVITY IN MOUSE SPINAL-CORD NEURONS IN CULTURE

被引：26

作者：

MICHIKAWA, M ^{[1
]}

KIKUCHI, S ^{[1
]}

KIM, SU ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA HOSP,DEPT MED,DIV NEUROL,VANCOUVER V6T 2B5,BC,CANADA

来源：

NEUROSCIENCE LETTERS | 1992年 / 140卷 / 01期

基金：

英国医学研究理事会;

关键词：

CHOLINE ACETYLTRANSFERASE; CHOLINERGIC NEURONAL DIFFERENTIATION FACTOR; LEUKEMIA INHIBITORY FACTOR; SPINAL CORD; TISSUE CULTURE;

D O I：

10.1016/0304-3940(92)90685-Z

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of leukemia inhibitory factor (LIF) on choline acetyltransferase (ChAT) enzyme activity in cultured mouse spinal cord neurons were examined. The administration of LIF to cultures at concentrations of 10 U/ml and higher enhanced ChAT activity approximately 3- to 4-fold in cultured spinal cord neurons. Among neurotrophic factors tested. basic fibroblast growth factor (bFGF) and insulin-like growth factor I (IGF-I) stimulated the development of ChAT activity but to a smaller extent than LIF, while interleukin 3 (IL-3), interleukin 6 (IL-6) and nerve growth factor (NGF) showed no apparent effect on ChAT development. Our results indicate that LIF, which has not been known to have any trophic effect on mammalian central nervous system neurons to date, acts as a potent differentiation factor for ChAT in cholinergic neurons of mouse spinal cord in culture.

引用

页码：75 / 77

页数：3

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