HELICOBACTER-PYLORI - ISOLATION FROM GASTRIC BIOPSY SPECIMENS

被引：4

作者：

MIRZA, SH ^{[1
]}

HANNAN, A ^{[1
]}

RIZVI, F ^{[1
]}

机构：

[1] ARMED FORCES INST PATHOL,RAWALPINDI,PAKISTAN

来源：

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY | 1993年 / 87卷 / 05期

关键词：

D O I：

10.1080/00034983.1993.11812799

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Forty-two gastric biopsies, in two transport media, were homogenized and cultured on three media under micro-aerophilic conditions. Brain-heart infusion agar with a commercial antibiotic supplement (giving 10 mg vancomycin, 5 mg trimethoprim and 2500 i.u. polymixin per litre) yielded the best results. Ordinary chocolate (heated) human blood agar could be used in laboratories with limited resources. Growth was obtained in 4-6 days at 37°C. All isolates were sensitive to metronidazole. The resistance to nalidixic acid and rapid urease production of Helicobacter pylori could be used to differentiate this species from Campylobacter spp. Indicator medium (brain-heart infusion broth with 7% human blood and 40mg 2,3,5-triphenyletetrazolium chloride agar/litre) also proved useful in identification.

引用

页码：483 / 486

页数：4

共 16 条

[1]

ARVIND AS, 1988, LANCET, V1, P704

[2]

Bizzozero G., 1893, ARCH MIKROSK ANAT, V42, P82, DOI [10.1007/BF02975307, DOI 10.1007/BF02975307]

[3] RAPID DIAGNOSIS OF CAMPYLOBACTER-PYLORIDIS ASSOCIATED GASTRITIS [J].