TRANSGENIC POTATO PLANTS EXPRESSING MAMMALIAN 2'-5' OLIGOADENYLATE SYNTHETASE ARE PROTECTED FROM POTATO VIRUS-X INFECTION UNDER FIELD CONDITIONS

被引：51

作者：

TRUVE, E

AASPOLLU, A

HONKANEN, J

PUSKA, R

MEHTO, M

HASSI, A

TERRI, TH

KELVE, M

SEPPANEN, P

SAARMA, M

机构：

[1] UNIV HELSINKI,INST BIOTECHNOL,POB 45,KARVAAMOKUJA 3A,SF-00014 HELSINKI,FINLAND

[2] ESTONIAN ACAD SCI,INST CHEM PHYS & BIOPHYS,TALLINN 0026,ESTONIA

[3] KEMIRA OY,RES CTR,SF-02271 ESPOO,FINLAND

[4] INST PLANT PROTECT,AGR RES CTR,SF-31600 JOKIOINEN,FINLAND

来源：

BIO-TECHNOLOGY | 1993年 / 11卷 / 09期

关键词：

D O I：

10.1038/nbt0993-1048

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We cloned and sequenced a rat cDNA encoding the 2'-5' oligoadenylate synthetase, a component of the mammalian interferon-induced antiviral response, and used Agrobacterium-mediated transformation to generate transgenic potato clones expressing this mammalian enzyme. In transgenic plants infected with potato virus X and followed under field conditions, virus concentrations in leaves and in tubers were significantly lower than in nontransgenic controls. Additionally, virus concentration in the leaves of five transgenic clones and in tubers of one clone was also lower than in transgenic potatoes expressing potato virus X coat protein.

引用

页码：1048 / 1052

页数：5

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