POLYETHYLENE GLYCOL-MODIFIED LIPASE CATALYZES ASYMMETRIC ALCOHOLYSIS OF DELTA-DECALACTONE IN N-DECANOL

被引：10

作者：

UEMURA, T

FURUKAWA, M

KODERA, Y

HIROTO, M

MATSUSHIMA, A

KUNO, H

MATSUSHITA, H

SAKURAI, K

INADA, Y

机构：

[1] TOIN UNIV YOKOHAMA,TOIN HUMAN SCI & TECHNOL CTR,DEPT MAT SCI & TECHNOL,AOBA KU,YOKOHAMA,KANAGAWA 225,JAPAN

[2] JAPAN TOBACCO INC,LIFE SCI RES LAB,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 01期

关键词：

D O I：

10.1007/BF00134197

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipase from Pseudomonas cepacia was modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine(activated PEG(2)) to form PEG-lipase. The PEG-lipase, soluble and active in organic solvents, catalyzes asymmetric alcoholysis of racemic delta-decalactone in alcohols to form (R)-5-hydroxydecanoic acid alkyl esters. The yield was 69% with 83% enantiomeric excess after 3 hr-reaction in n-decanol at 50 degrees C. The advantage of this reaction is that the alcoholysis proceeds efficiently in straight hydrophobic substrates without any organic solvents.

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页码：61 / 66

页数：6

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