VICIA-FABA CHROMOSOMAL ABERRATION ASSAY

被引：92

作者：

KANAYA, N

GILL, BS

GROVER, IS

MURIN, A

OSIECKA, R

SANDHU, SS

ANDERSSON, HC

机构：

[1] ENVIRONM HLTH RES & TESTING INC, RES TRIANGLE PK, NC 27709 USA

[2] GURU NANAK DEV UNIV, DEPT BOT SCI, AMRITSAR 143005, PUNJAB, INDIA

[3] COMENIUS UNIV BRATISLAVA, INST MOLEC & SUBCELLULAR BIOL, KARYOL LAB, BRATISLAVA 81102, SLOVAKIA

[4] UNIV LODZ, PLANT CYTOL & CYTOCHEM LAB, PL-90237 LODZ, POLAND

[5] US EPA, HLTH EFFECTS RES LAB, RES TRIANGLE PK, NC 27711 USA

[6] US EPA, ATMOSPHER RES & EXPOSURE ASSESSMENT LABS, RES TRIANGLE PK, NC 27711 USA

[7] NATL FOOD ADM TOXICOL LAB, S-75126 UPPSALA, SWEDEN

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1994年 / 310卷 / 02期

关键词：

IPCS COLLABORATIVE STUDY; VICIA FABA; CLASTOGENS; CHROMOSOMAL ABERRATIONS; MALEIC HYDRAZIDE; 3-AZIDOGLYCEROL; N-METHYL-N-NITROSOUREA; SODIUM AZIDE;

D O I：

10.1016/0027-5107(94)90116-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A collaborative study involving laboratories in six countries was initiated under the sponsorship of the International Programme on Chemical Safety (IPCS) to determine the sensitivity, efficiency and reliability of the Vicia faba root tip meristem chromosomal aberration assay using a standardized protocol. The six laboratories that participated in this study were located in the Slovak Republic, India, Japan, Poland, Sweden and the USA. All laboratories adhered to a standardized protocol for the Vicia faba chromosomal aberration assay. Four coded chemicals, azidoglycerol (AG), N-methyl-N-nitrosourea (MNU), sodium azide (NaN3) and maleic hydrazide (MH) were tested with the Vicia faba chromosomal aberration assay. Of the four chemicals, three (MH, AG and MNU) were found to be clastogenic and gave a concentration related response. However, the results of NaN3 were equivocal which might be explained by the stability of NaN3. The conclusions from this study suggest that the Vicia faba chromosomal aberration bioassay is an efficient and reliable short-term bioassay for the rapid screening of chemicals for clastogenicity.

引用

页码：231 / 247

页数：17

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