HISTOCHEMICAL-DEMONSTRATION OF DELTA-5,3-BETA-HYDROXYSTEROID DEHYDROGENASE-ACTIVITY IN CULTURED LEYDIG-CELLS UNDER THE INFLUENCE OF GONADOTROPIC-HORMONES AND TESTOSTERONE

被引：25

作者：

BILINSKA, B

机构：

[1] Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków

来源：

INTERNATIONAL JOURNAL OF ANDROLOGY | 1979年 / 2卷 / 05期

关键词：

3β‐OH‐SDH; culture; gonadotropic hormones; Leydig cells; testosterone; Δ [!sup]5[!/sup;

D O I：

10.1111/j.1365-2605.1979.tb00072.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

The influence of gonadotropic hormones or testosterone upon activity of Δ5, 3β‐hydroxysteroid dehydrogenase (Δ5,3β‐OH‐SDH) within cultured Leydig cells was histochemically investigated. The following amounts of hormones were added into the culture medium: LH – 10, 100, 500, and 1000 ng/ml, FSH – 100 ng/ml, Testosterone (T) – 150 ng/ml of culture medium. Doses 10 and 100 ng LH stimulated the activity of the histochemically investigated enzyme, while 500 ng decreased enzyme activity and 1000 ng failed to support culture at all. FSH did not exert any influence on enzyme activity of cultured cells while testosterone decreased its activity beginning on day 6 in culture. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：385 / 394

页数：10

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