DESIGN OF 2 IMMOBILIZED CELL CATALYSTS BY ENTRAPMENT ON GELATIN - INTERNAL DIFFUSION ASPECTS

被引：16

作者：

CASTILLO, E ^{[1
]}

RODRIGUEZ, M ^{[1
]}

CASAS, L ^{[1
]}

QUINTERO, R ^{[1
]}

LOPEZMUNGUIA, A ^{[1
]}

机构：

[1] UNIV NACL AUTONOMA MADRID,CTR INVEST INGN GENET & BIOTECNOL,APARTADO POSTAL 510-3,CUERNAVACA 62271,MORELOS,MEXICO

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 02期

关键词：

IMMOBILIZED CELLS; MASS TRANSFER EFFECTS; LACTASE; PENICILLIN ACYLASE;

D O I：

10.1016/0141-0229(91)90167-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Experimental results obtained during the design of two immobilized cell catalysts by entrapment on gelatin are presented. Strong diffusional limitations are found and explained with the usual parameters and models, introducing an empirical correlation between substrate concentration and effectiveness factor. The effect of particle size, enzyme load, and specific activity in the system is discussed in terms of cooperation between bioengineers and geneticists.

引用

页码：127 / 133

页数：7

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