RETROVIRAL-MEDIATED TRANSFER OF A DYSTROPHIN MINIGENE INTO MDX MOUSE MYOBLASTS INVITRO

被引：64

作者：

DUNCKLEY, MG

LOVE, DR

DAVIES, KE

WALSH, FS

MORRIS, GE

DICKSON, G

机构：

[1] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,GUYS HOSP,DEPT EXPTL PATHOL,LONDON BRIDGE,LONDON SE1 9RT,ENGLAND

[2] JOHN RADCLIFFE HOSP,INST MOLEC MED,MOLEC GENET GRP,OXFORD OX3 9DU,ENGLAND

[3] NE WALES INST,DIV RES,DEESIDE CH5 4BR,CLWYD,WALES

来源：

FEBS LETTERS | 1992年 / 296卷 / 02期

基金：

英国医学研究理事会;

关键词：

DYSTROPHIN; RECOMBINANT RETROVIRUS; GENE TRANSFER; MDX MOUSE; SKELETAL MUSCLE;

D O I：

10.1016/0014-5793(92)80363-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have demonstrated expression of a 6.3 kb Becker muscular dystrophy (BMD) human dystrophin cDNA following retroviral-mediated transduction of cultured myoblasts from the dystrophin-deficient mdx mouse. The truncated dystrophin protein was localised to the sarcolemma of differentiated myotubes by antibodies against the C-terminus of the molecule, and produced an identical immunostaining pattern to that observed in control myotubes expressing normal endogenous dystrophin. These results indicate that retroviral-mediated gene transfer may be useful for experimental in vivo studies on the complementation of dystrophin gene mutations.

引用

页码：128 / 134

页数：7

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