TRANSFORMATION AND REGENERATION OF BRASSICA-RAPA USING AGROBACTERIUM-TUMEFACIENS

被引：39

作者：

RADKE, SE ^{[1
]}

TURNER, JC ^{[1
]}

FACCIOTTI, D ^{[1
]}

机构：

[1] CALGENE INC,1920 5TH ST,DAVIS,CA 95616

来源：

PLANT CELL REPORTS | 1992年 / 11卷 / 10期

关键词：

BRASSICA; OILSEED RAPE; GENE TRANSFER; TRANSGENIC PLANTS; SILVER NITRATE;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Transformation and regeneration procedures for obtaining transgenic Brassica rapa ssp. oleifera plants are described. Regeneration frequencies were increased by using silver nitrate and by adjusting the duration of exposure to 2,4-D. For transformation, Agrobacterium tumefaciens strain EHA101 containing a binary plasmid with the neomycin phosphotransferase gene (NPT II) and the b-glucuronidase gene (GUS) was cocultivated with hypocotyl explants from the oilseed B. rapa cvs. Tobin and Emma. Transformed plants were obtained within three months of cocultivation. Transformation frequencies for the cultivars Tobin and Emma were 1-9%. Evidence for transformation was shown by NPT II dot blot assay, the GUS fluorometric assay, Southern analysis, and segregation of the kanamycin-resistance trait in the progeny. The transformation and regeneration procedure described here has been used routinely to transform two cultivars of B. rapa and 18 cultivars of B. napus.

引用

页码：499 / 505

页数：7

共 24 条

[1] ACCUMULATION OF A BRAZIL NUT ALBUMIN IN SEEDS OF TRANSGENIC CANOLA RESULTS IN ENHANCED LEVELS OF SEED PROTEIN METHIONINE [J].