TRANSFORMATION AND REGENERATION OF BRASSICA-RAPA USING AGROBACTERIUM-TUMEFACIENS

被引:39
作者
RADKE, SE [1 ]
TURNER, JC [1 ]
FACCIOTTI, D [1 ]
机构
[1] CALGENE INC,1920 5TH ST,DAVIS,CA 95616
关键词
BRASSICA; OILSEED RAPE; GENE TRANSFER; TRANSGENIC PLANTS; SILVER NITRATE;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transformation and regeneration procedures for obtaining transgenic Brassica rapa ssp. oleifera plants are described. Regeneration frequencies were increased by using silver nitrate and by adjusting the duration of exposure to 2,4-D. For transformation, Agrobacterium tumefaciens strain EHA101 containing a binary plasmid with the neomycin phosphotransferase gene (NPT II) and the b-glucuronidase gene (GUS) was cocultivated with hypocotyl explants from the oilseed B. rapa cvs. Tobin and Emma. Transformed plants were obtained within three months of cocultivation. Transformation frequencies for the cultivars Tobin and Emma were 1-9%. Evidence for transformation was shown by NPT II dot blot assay, the GUS fluorometric assay, Southern analysis, and segregation of the kanamycin-resistance trait in the progeny. The transformation and regeneration procedure described here has been used routinely to transform two cultivars of B. rapa and 18 cultivars of B. napus.
引用
收藏
页码:499 / 505
页数:7
相关论文
共 24 条
[1]   ACCUMULATION OF A BRAZIL NUT ALBUMIN IN SEEDS OF TRANSGENIC CANOLA RESULTS IN ENHANCED LEVELS OF SEED PROTEIN METHIONINE [J].
ALTENBACH, SB ;
KUO, CC ;
STARACI, LC ;
PEARSON, KW ;
WAINWRIGHT, C ;
GEORGESCU, A ;
TOWNSEND, J .
PLANT MOLECULAR BIOLOGY, 1992, 18 (02) :235-245
[2]  
BARFIELD DG, 1991, PLANT CELL REP, V10, P308, DOI 10.1007/BF00193148
[3]   ETHYLENE INHIBITORS ENHANCED DENOVO SHOOT REGENERATION FROM COTYLEDONS OF BRASSICA-CAMPESTRIS SSP CHINENSIS (CHINESE CABBAGE) INVITRO [J].
CHI, GL ;
PUA, EC .
PLANT SCIENCE, 1989, 64 (02) :243-250
[4]  
CHI GL, 1990, PLANT CELL REP, V9, P195, DOI 10.1007/BF00232178
[5]   NOVEL AND USEFUL PROPERTIES OF A CHIMERIC PLANT PROMOTER COMBINING CAMV-35S AND MAS ELEMENTS [J].
COMAI, L ;
MORAN, P ;
MASLYAR, D .
PLANT MOLECULAR BIOLOGY, 1990, 15 (03) :373-381
[6]   TRANSFORMATION OF BRASSICA-NAPUS AND BRASSICA-OLERACEA USING AGROBACTERIUM-TUMEFACIENS AND THE EXPRESSION OF THE BAR AND NEO GENES IN THE TRANSGENIC PLANTS [J].
DEBLOCK, M ;
DEBROUWER, D ;
TENNING, P .
PLANT PHYSIOLOGY, 1989, 91 (02) :694-701
[7]  
Dellaporta SL, 1983, PLANT MOL BIOL REP, V1, P19, DOI [DOI 10.1007/BF02712670, 10.1007/BF02712670]
[8]  
FACCIOTTI D, 1989, 5TH P CRUC GEN WORKS, P48
[9]   TRANSFORMATION OF BRASSICA-NAPUS WITH AGROBACTERIUM-TUMEFACIENS BASED VECTORS [J].
FRY, J ;
BARNASON, A ;
HORSCH, RB .
PLANT CELL REPORTS, 1987, 6 (05) :321-325
[10]  
HACHEY JE, 1991, PLANT CELL REP, V9, P549, DOI 10.1007/BF00232329