ISOLATION OF 2 PYRUVATE-KINASE ACTIVITIES IN THE PARASITIC PROTOZOAN LEISHMANIA-MEXICANA-AMAZONENSIS

被引：7

作者：

PONTESUCRE, A

ALONSO, G

MARTINEZ, C

HUNG, A

RIVAS, L

RAMIREZ, JL

机构：

[1] CENT UNIV VENEZUELA, FAC CIENCIAS, CTR BIOL CELULAR, CARACAS, VENEZUELA

[2] CSIC, CTR INVEST BIOL, MADRID, SPAIN

[3] ESCUELA MED LUIS RAZETTI, INST MED EXPTL, CATEDRA FISIOL, CARACAS, VENEZUELA

[4] ESCUELA MED JM VARGAS, FAC MED, CATEDRA BIOQUIM, CARACAS, VENEZUELA

[5] CENT UNIV VENEZUELA, FAC CIENCIAS, CTR BIOL CELULAR, CARACAS, VENEZUELA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1993年 / 300卷 / 01期

关键词：

D O I：

10.1006/abbi.1993.1063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using phosphocellulose affinity chromatography we were able to separate two pyruvate kinase (EC 2.7.1.40) activities in the parasitic protozoan Leishmania mexicana amazonesis. One activity (PYK1) showed hyperbolic kinetics and was decreased by fructose 2,6-bisphosphate, whereas the second activity (PYK2) showed sigmoidal kinetics for the substrate phosphoenolpyruvate and was activated by fructose 2,6-bisphosphate. Molecular sieve chromatography (Sephacryl S-400) of PYK1 produced a single peak of apparent molecular mass around 200,000, while PYK2 eluted at a position corresponding to M(r) 55,000. © 1993 Academic Press, Inc.

引用

页码：466 / 471

页数：6

共 24 条

[1]

ADROHER FJ, 1990, MOL CELL BIOCHEM, V94, P71, DOI 10.1007/BF00223564

[2] MOLECULAR-CLONING AND ANALYSIS OF 2 TANDEMLY LINKED GENES FOR PYRUVATE-KINASE OF TRYPANOSOMA-BRUCEI [J].

ALLERT, S ;

ERNEST, I ;

POLISZCZAK, A ;

OPPERDOES, FR ;

MICHELS, PAM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 200 (01) :19-27

[3] PURIFICATION IN A SINGLE STEP AND KINETIC CHARACTERIZATION OF THE PYRUVATE-KINASE OF TRYPANOSOMA-BRUCEI [J].

BARNARD, JP ;

PEDERSEN, PL .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 31 (02) :141-147

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5]

BUCHER T, 1962, METHOD ENZYMOL, V1, P141

[6] CHARACTERIZATION OF PYRUVATE-KINASE OF TRYPANOSOMA-BRUCEI AND ITS ROLE IN THE REGULATION OF CARBOHYDRATE-METABOLISM [J].

CALLENS, M ;

KUNTZ, DA ;

OPPERDOES, FR .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 47 (01) :19-29

[7] THE AEROBIC FERMENTATION OF GLUCOSE BY TRYPANOSOMA-CRUZI [J].

CANNATA, JJB ;

CAZZULO, JJ .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1984, 79 (03) :297-308

[8] END PRODUCTS AND ENZYME LEVELS OF AEROBIC GLUCOSE FERMENTATION IN TRYPANOSOMATIDS [J].

CAZZULO, JJ ;

DECAZZULO, BMF ;

ENGEL, JC ;

CANNATA, JJB .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1985, 16 (03) :329-343

[9] COMPARATIVE STUDIES ON PHYSICAL-PROPERTIES CONCERNED WITH THE STABILITY OF UNPHOSPHORYLATED, PHOSPHORYLATED AND PROTEOLYTICALLY MODIFIED L-TYPE PYRUVATE-KINASE FROM PIG-LIVER [J].

DAHLQVISTEDBERG, U .

ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY, 1984, 38 (04) :267-272

[10] PURIFICATION AND CHARACTERIZATION OF A METABOLITE-REGULATED PYRUVATE-KINASE FROM LEISHMANIA-MAJOR PROMASTIGOTES [J].

ETGES, R ;

MUKKADA, AJ .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 27 (2-3) :281-290

← 1 2 3 →