SENSITIVE ASSAY FOR DETECTION OF HEPATOCELLULAR-CARCINOMA ASSOCIATED GENE-TRANSCRIPTION (ALPHA-FETOPROTEIN MESSENGER-RNA) IN BLOOD

被引：35

作者：

MATSUMURA, M ^{[1
]}

NIWA, Y ^{[1
]}

HIKIBA, Y ^{[1
]}

OKANO, KI ^{[1
]}

KATO, N ^{[1
]}

SHIINA, S ^{[1
]}

SHIRATORI, Y ^{[1
]}

OMATA, M ^{[1
]}

机构：

[1] ASAHI LIFE FDN,INST ADULT DIS,DIV GASTROENTEROL & HEPATOL,SHINJUKU KU,TOKYO,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 207卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1259

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sensitivity of RT-PCR and nested RT-PCR for detection of circulating hepatocellular carcinoma was assessed by demonstrating the tumor cell-associated gene transcription, alpha-fetoprotein mRNA, in the nuclear cells of peripheral blood. When HepG2 cells were mixed in blood, 100-1000 tumor cells/5ml of blood could be detected by RT-PCR, in contrast to 1-10 tumor cells/5ml of blood by nested RT-PCR. In addition, 2x10(4) copies of AFP mRNA were found in one HepG2 cell when analyzed by the quantitative nested RT-PCR assay. Thus, the nested RT-PCR assay could provide a useful tool for detecting a tiny amount of circulating tumor cells in patients with hepatocellular carcinoma presenting extra-hepatic metastasis. (C) 1995 Academic Press, Inc.

引用

页码：813 / 818

页数：6

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