PURIFICATION OF NADPH - CYTOCHROME-C (CYTOCHROME-P-450) REDUCTASE FROM HAMSTER LIVER-MICROSOMES BY DETERGENT EXTRACTION AND AFFINITY-CHROMATOGRAPHY

被引：54

作者：

ARDIES, CM

LASKER, JM

BLOSWICK, BP

LIEBER, CS

机构：

[1] VET ADM MED CTR,CTR ALCOHOL RES & TREATMENT,BRONX,NY 10468

[2] CUNY MT SINAI SCH MED,NEW YORK,NY 10029

来源：

ANALYTICAL BIOCHEMISTRY | 1987年 / 162卷 / 01期

关键词：

D O I：

10.1016/0003-2697(87)90008-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：39 / 46

页数：8

共 20 条

[1] PITFALLS IN THE USE OF COMMERCIAL NON-IONIC DETERGENTS FOR THE SOLUBILIZATION OF INTEGRAL MEMBRANE-PROTEINS - SULFHYDRYL OXIDIZING CONTAMINANTS AND THEIR ELIMINATION [J].

CHANG, HW ;

BOCK, E .

ANALYTICAL BIOCHEMISTRY, 1980, 104 (01) :112-117

[2] NADPH-CYTOCHROME P-450 REDUCTASE FROM RAT-LIVER - PURIFICATION BY AFFINITY CHROMATOGRAPHY AND CHARACTERIZATION [J].

DIGNAM, JD ;

STROBEL, HW .

BIOCHEMISTRY, 1977, 16 (06) :1116-1123

[3] RAPID MICROMETHOD FOR DETERMINATION OF FMN AND FAD IN MIXTURES [J].

FAEDER, EJ ;

SIEGEL, LM .

ANALYTICAL BIOCHEMISTRY, 1973, 53 (01) :332-336

[4] PROPERTIES OF NADPH-CYTOCHROME P-450 REDUCTASE PURIFIED FROM RABBIT LIVER-MICROSOMES [J].

FRENCH, JS ;

COON, MJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1979, 195 (02) :565-577

[5]

GORNALL AG, 1949, J BIOL CHEM, V177, P741

[6] SOME PROPERTIES OF HEPATIC REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE - CYTOCHROME-C REDUCTASE [J].

IYANAGI, T ;

MASON, HS .

BIOCHEMISTRY, 1973, 12 (12) :2297-2308

[7] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9] RECONSTITUTED MAMMALIAN MIXED-FUNCTION OXIDASES - REQUIREMENTS, SPECIFICITIES AND OTHER PROPERTIES [J].

LU, AYH ;

WEST, SB .

PHARMACOLOGY & THERAPEUTICS PART A-CHEMOTHERAPY TOXICOLOGY AND METABOLIC INHIBITORS, 1978, 2 (02) :337-358

[10]

LU AYH, 1969, J BIOL CHEM, V244, P3714

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