ANTIBODY-COATED PROTEIN A-BEARING STAPHYLOCOCCUS-AUREUS - VERSATILE AND STABLE IMMUNE REAGENT

被引：30

作者：

NATALI, PG ^{[1
]}

PELLEGRINO, MA ^{[1
]}

WALKER, L ^{[1
]}

FERRONE, S ^{[1
]}

REISFELD, RA ^{[1
]}

机构：

[1] SCRIPPS CLIN & RES FDN,DEPT MOLEC IMMUNOL,LA JOLLA,CA 92037

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 25卷 / 03期

关键词：

D O I：

10.1016/0022-1759(79)90113-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The human β2-microglobulin antigen-antibody system was used as a model to illustrate the versatility of a microradioimmunoassay technique using protein A-bearing Staphylococcus aureus Cowan I strain (SACI) bacteria as a non-specific immunoadsorbent in place of a second antibody. Experimental conditions are described for a sensitive microassay which makes it possible to process large numbers of samples more rapidly and with minimum handling. Furthermore, SACI coated with specific antibodies by mixing with unfractionated antisera are a versatile reagent not only for radioimmunoassay but also for use as molecular probes to characterize cell surface antigens. Antibody-coated SACI could be lyophilized and proved extremely stable in storage thus providing a unique advantage for use in binding inhibition assays and as versatile reagent for clinical and investigative immunology. © 1979.

引用

页码：255 / 264

页数：10

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