ADHERENCE OF HUMAN NEUTROPHILS CHANGES CA-2+ SIGNALING DURING ACTIVATION WITH OPSONIZED PARTICLES

被引：9

作者：

KOENDERMAN, L

TOOL, ATJ

HOOYBRINK, B

ROOS, D

HANSEN, CA

WILLIAMSON, JR

VERHOEVEN, AJ

机构：

[1] NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,POB 9406,1006 AK AMSTERDAM,NETHERLANDS

[2] UNIV AMSTERDAM,EXPTL & CLIN IMMUNOL LAB,AMSTERDAM,NETHERLANDS

[3] UNIV PENN,SCH MED,DEPT BIOCHEM & BIOPHYS,PHILADELPHIA,PA 19104

来源：

FEBS LETTERS | 1990年 / 270卷 / 1-2期

关键词：

Adherence; Ca[!sup]2+[!/sup] signaling; Neutrophil;

D O I：

10.1016/0014-5793(90)81232-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in the cytosolic free Ca2+ concentration ([Ca2+]i) upon activation of human neutrophils by opsonized particles (serum-treated zymosan; STZ) were evaluated by three different methods: (i) measurement of total fluorescence changes in indo-1 loaded neutrophils activated in suspension; (ii) measurement of fluorescence changes in individual indo-1 loaded neutrophils in a flow cytometer and (iii) measurement of fluorescence changes in individual fura-2 loaded neutrophils adherent to serum-coated coverslips. Our study shows that the opsonized particle-induced change in [Ca2+]i in neutrophils is altered during adherence of the cells to a serum-coated surface. These observations might be of importance for neutrophil function in vivo, since adherence is a prerequisite for diapedesis and chemotaxis. © 1990.

引用

页码：49 / 52

页数：4

共 26 条

[1] THE SUPEROXIDE-FORMING ENZYMATIC SYSTEM OF PHAGOCYTES [J].