STRUCTURES DERIVED FROM COWPEA CHLOROTIC MOTTLE AND BROME MOSAIC VIRUS PROTEIN

被引:75
作者
BANCROFT, JB
BRACKER, CE
WAGNER, GW
机构
[1] Department of Botany and Plant Pathology, Purdue University, Lafayette, IN 47907, Lilly Hall of Life Sciences
基金
美国国家科学基金会;
关键词
D O I
10.1016/0042-6822(69)90374-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The behavior at different salt and acidic pH levels of protein isolated from three spherical plant viruses was investigated. Broad bean mottle virus protein did not aggregate into recognizable organized structures. Protein from brome mosaic virus formed capsids similar to those of the virus at a pH and salt-dependent efficiency with best yields at pH 5.0 in 0.2 M NaCl. Best yields for cowpea chlorotic mottle virus protein capsids occurred at pH 5.0 in 0.1 M NaCl or from pH 3.5 to 5.0 in 0.2 M NaCl. Protein from the latter virus also formed double-shelled and rosettelike particles in 0.2 M NaCl from pH 5.3 to 5.7. Narrow tubes were made at pH levels of 6.0 and higher, and the tubes were accompanied by T = 1 and T = 3 particles if ribonu-clease-digested RNA was present. In the absence of NaCl in 0.01 M acetate buffer at pH 4.0 and 4.5, laminar and platelike aggregates were found with CCMV protein. The production and some of the properties of the various forms are described. © 1969.
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页码:324 / &
相关论文
共 13 条
[1]   SELF-ASSEMBLY OF A NUCLEIC-ACID FREE PSEUDO-TOP COMPONENT FOR A SMALL SPHERICAL VIRUS [J].
BANCROFT, JB ;
WAGNER, GW ;
BRACKER, CE .
VIROLOGY, 1968, 36 (01) :146-&
[2]   A STUDY OF SELF-ASSEMBLY PROCESS IN A SMALL SPHERICAL VIRUS - FORMATION OF ORGANIZED STRUCTURES FROM PROTEIN SUBUNITS IN VITRO [J].
BANCROFT, JB ;
HILLS, GJ ;
MARKHAM, R .
VIROLOGY, 1967, 31 (02) :354-&
[3]   MOLECULAR WEIGHT AND OTHER BIOPHYSICAL PROPERTIES OF BROMEGRASS MOSAIC VIRUS [J].
BOCKSTAHLER, LE ;
KAESBERG, P .
BIOPHYSICAL JOURNAL, 1962, 2 (01) :1-&
[4]   PHYSICAL PRINCIPLES IN CONSTRUCTION OF REGULAR VIRUSES [J].
CASPAR, DLD ;
KLUG, A .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1962, 27 :1-&
[5]   STRUCTURE OF REAGGREGATED PROTEIN SHELLS OF 2 SPHERICAL VIRUSES [J].
FINCH, JT ;
BANCROFT, JB .
NATURE, 1968, 220 (5169) :815-&
[6]  
HERMANN R, 1968, BIOCHEM BIOPH RES CO, V30, P576
[7]   ASSEMBLY IN VITRO OF SOME SMALL SPHERICAL VIRUSES HYBRID VIRUSES AND OTHER NUCLEOPROTEINS [J].
HIEBERT, E ;
BANCROFT, JB ;
BRACKER, CE .
VIROLOGY, 1968, 34 (03) :492-&
[8]   A STUDY OF TUBES PRODUCED IN PLANTS INFECTED WITH A STRAIN OF TURNIP YELLOW MOSAIC VIRUS [J].
HITCHBORN, JH ;
HILLS, GJ .
VIROLOGY, 1968, 35 (01) :50-+
[9]  
HITCHBORN JH, 1965, VIROLOGY, V26, P756
[10]  
INCARDONA HL, 1964, BIOPHYSICAL J, V4, P11