HIGH-EFFICIENCY TRANSFORMATION SYSTEM FOR THE BIOCONTROL AGENTS, TRICHODERMA SPP

被引：67

作者：

HERRERAESTRELLA, A ^{[1
]}

GOLDMAN, GH ^{[1
]}

VANMONTAGU, M ^{[1
]}

机构：

[1] STATE UNIV GHENT,GENET LAB,B-9000 GHENT,BELGIUM

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 05期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb00654.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed an efficient transformation system based on the use of polyethylene glycol and CaCl2 for the biocontrol agents, Trichoderma spp. Trans formation was obtained with the plasmid pAN7‐1, carrying a bacterial hygromycin‐resistance gene as a selectable marker, under the control of Aspergillus nidulans heterologous expression signals. The system described here yielded 200‐800 transformants per μg of DNA. Transformants contained several copies of the plasmid integrated into their genome, apparently at the same site in the different transformants analysed. Stability of the transformants was achieved by inserting a 2.4kb homologous DNA fragment into pAN7‐1. Southern blot analysis indicated that integration in the stable transformants occurs through non‐homologous recombination. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：839 / 843

页数：5

共 19 条

[1] TRANSFORMATION OF ASPERGILLUS-NIDULANS BY THE OROTIDINE-5'-PHOSPHATE DECARBOXYLASE GENE OF NEUROSPORA-CRASSA
BALLANCE, DJ
BUXTON, FP
TURNER, G
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 112 (01) : 284 - 289
[2] ISOLATION OF PROTOPLASTS FROM ASPERGILLUS-NIDULANS CONIDIOSPORES
BOS, CJ
SLAKHORST, SM
[J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1981, 27 (04) : 400 - 407
[3] HIGH-FREQUENCY TRANSFORMATION OF PENICILLIUM-CHRYSOGENUM
CANTORAL, JM
DIEZ, B
BARREDO, JL
ALVAREZ, E
MARTIN, JF
[J]. BIO-TECHNOLOGY, 1987, 5 (05): : 494 - 497
[4] EFFICIENT TRANSFORMATION OF NEUROSPORA-CRASSA BY UTILIZING HYBRID PLASMID DNA
CASE, ME
SCHWEIZER, M
KUSHNER, SR
GILES, NH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (10) : 5259 - 5263
[5] Chet I., 1987, INNOVATIVE APPROACHE, V1, P137
[6] ANTAGONISTIC PROPERTIES OF SPECIES-GROUPS OF TRICHODERMA .1. PRODUCTION OF NON-VOLATILE ANTIBIOTICS
DENNIS, C
WEBSTER, J
[J]. TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1971, 57 (AUG): : 25 - &
[7] ANTAGONISTIC PROPERTIES OF SPECIES-GROUPS OF TRICHODERMA .2. PRODUCTION OF VOLATILE ANTIBIOTICS
DENNIS, C
WEBSTER, J
[J]. TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1971, 57 (AUG): : 41 - +
[8] DEGRADATION OF PLANT PATHOGENIC FUNGI BY TRICHODERMA-HARZIANUM
ELAD, Y
CHET, I
HENIS, Y
[J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1982, 28 (07) : 719 - 725
[9] Huiet L., 1985, GENE MANIPULATION FU, P229
[10] Maniatis T., 1982, MOL CLONING

← 1 2 →