PRODUCTION OF A SPECIFIC MONOCLONAL-ANTIBODY FOR THE IDENTIFICATION OF LEISHMANIA-(LEISHMANIA)-VENEZUELENSIS

被引：11

作者：

HANHAM, CA ^{[1
]}

SHAW, JJ ^{[1
]}

LAINSON, R ^{[1
]}

MCMAHONPRATT, D ^{[1
]}

机构：

[1] INST EVANDRO CHAGAS FUNDACAO SESP, WELLCOME PARASITOL UNIT, BELEM, BRAZIL

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1990年 / 42卷 / 05期

关键词：

D O I：

10.4269/ajtmh.1990.42.453

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We describe a monoclonal antibody (Mab), V1, specific for Leishmania (Leishmania) venezuelensis. Previous Mabs and DNA probes were not specific for this parasite, and so it was not directly possible to distinguish L. (L.) venezuelensis from other Leishmania species. Immunofluorescent staining using Mabs may be performed on very few parasites, whereas other methods of identification usually require far greater numbers of organisms. L. (L.) venezuelensis frequently dies on subculture. Mab V1 can be used to identify this parasite by indirect immunofluorescence and radioimmunoassay.

引用

页码：453 / 459

页数：7

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