CHARACTERIZATION OF CRYSTALS OF A FAB FRAGMENT OF A MURINE MONOCLONAL-ANTIBODY

被引：2

作者：

BAN, N ^{[1
]}

ESCOBAR, C ^{[1
]}

DAY, J ^{[1
]}

GREENWOOD, A ^{[1
]}

LARSON, S ^{[1
]}

MCPHERSON, A ^{[1
]}

机构：

[1] IMMUNOPHARMACEUT INC,SAN DIEGO,CA 92127

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 222卷 / 03期

关键词：

CRYSTALS; FAB; X-RAY; ANTIBODY;

D O I：

10.1016/0022-2836(91)90484-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Fab fragment of an antibody, made against an E2-specific feline infectious peritonitis virus neutralizing antibody, has been crystallized in a form suitable for X-ray diffraction analysis from PEG 4000 using vapor diffusion methods. The Fab fragment crystals diffract to about 2·9 Å resolution and are of triclinic space group P1. Unit cell dimensions, by which the reciprocal lattice can be indexed, are a = 57·16 A ̊, b = 70·85 A ̊, c = 75·81 A ̊, α = 85·11{ring operator}, β = 121·28{ring operator} and γ = 116·33{ring operator}. There are two Fab fragments comprising the asymmetric unit of the crystals. The presence of a pseudo-mirror plane in the diffraction pattern suggests the presence of at least an approximate dyad axis relating the two Fab fragments within the asymmetric unit. © 1991.

引用

页码：445 / 446

页数：2

共 5 条

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