EFFECT OF REDOX POTENTIAL ON METHANOGENESIS BY METHANOSARCINA-BARKERI

Concentrations of 0.5% O2 immediately inhibited CH4 production from methanol by Methanosarcina barkeri. Simultaneously, the redox potential of the medium increased to about + 100 mV. However, the rates of CH4 production were not significantly affected, when the redox potential of an anoxic medium was adjusted to values between - 420 mV and + 100 mV by addition of titanium(III) citrate, sodium dithionite, flavin adenine dinucleotide, or sodium ascorbate. When the redox potential was adjusted to values between - 80 mV and + 550 mV by means of mixtures of ferrocyanide and ferricyanide, CH4 production was not inhibited until a redox potential of about + 420 mV was reached. M. barkeri was able to reduce 0.5 mM ferricyanide solution at + 430 mV within < 30 min to a value of about + 50 mV, and then to start CH4 production. Higher ferricyanide concentrations were only partially reduced. The extent of reduction of ferricyanide was also dependent on the substrate concentration (methanol) and the density of the bacterial suspension. The results show that M. barkeri was able to generate to a certain extent by itself the redox environment which suited the production of CH4. However, the bacteria probably have not enough reducing power to decrease the redox potential below the critical level of + 50 mV, if O2 is present at concentrations > 0.005%.