INTERACTION BETWEEN HSP70 AND HSP40, EUKARYOTIC HOMOLOGS OF DNAK AND DNAJ, IN HUMAN-CELLS EXPRESSING MUTANT-TYPE P53

被引：55

作者：

SUGITO, K

YAMANE, M

HATTORI, H

HAYASHI, Y

TOHNAI, I

UEDA, M

TSUCHIDA, N

OHTSUKA, K

机构：

[1] AICHI CANC CTR,RES INST,EXPTL RADIOL LAB,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

[2] NAGOYA UNIV,SCH MED,DEPT ORAL SURG,SHOWA KU,NAGOYA,AICHI 466,JAPAN

[3] TOKYO MED & DENT UNIV,FAC DENT,DEPT MOLEC CELLULAR ONCOL & MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

FEBS LETTERS | 1995年 / 358卷 / 02期

关键词：

HSP70 (DNAK); HSP40 (DNAJ); MOLECULAR CHAPERONE; P53; IMMUNOPRECIPITATION;

D O I：

10.1016/0014-5793(94)01417-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently identified a novel 40-kDa heat-shock protein hsp40 as a mammalian homologue of bacterial DnaJ protein. Here we demonstrate the physical interaction between hsp70 (DnaK homologue) and hsp40 in human cells as determined by immunoprecipitation methods. Co-immunoprecipitation of hsp70 with hsp40 was dependent on the presence of ATP or unfolded protein (reduced carboxymethylated alpha-lactalbumin). A mutant type of tumor suppressor gene product, mtp53, was co-immunoprecipitated not only with hsp70 but also with hsp40. These results suggest the existence of a hsp70(DnaK)/hsp40(DnaJ) chaperone system in mammalian cells.

引用

收藏

页码：161 / 164

页数：4

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