THE EFFECT OF IGG AGGREGATE SIZE AND CONCENTRATION ON REACTIVITY IN IMMUNE-COMPLEX ASSAYS

被引：22

作者：

MCCARTHY, D ^{[1
]}

GODDARD, DH ^{[1
]}

HOLBOROW, EJ ^{[1
]}

HORSFALL, AC ^{[1
]}

MUMFORD, PA ^{[1
]}

MAINI, RN ^{[1
]}

机构：

[1] UNIV LONDON LONDON HOSP, COLL MED, MRC, IMMUNOL GRP, BONE & JOINT RES UNIT, LONDON E1 2AD, ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1981年 / 47卷 / 03期

关键词：

D O I：

10.1016/0022-1759(81)90291-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The reactivity of heat-aggregated IgG of known size, in the [human Burkitt''s lymphoma] Raji cell assay, the [complement] C1q binding assay and the C1q solid phase radioimmunoassay as a function of concentration, was investigated. Marked differences were found in the way that the 3 assays behave when IgG concentration and aggregate size varied. These findings indicate the pitfalls in attempting to express the results of immune complex assays performed on biological fluids in terms of equivalent concentrations of aggregated IgG.

引用

页码：349 / 358

页数：10

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